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The sequence coverage cleavage map for the +10, +12, and +14 charge state precursors of β-lactoglobulin are shown. The left sequence coverage values represent the region of the protein which does not contain any disulfide bonds and the left values show the sequence coverage which contains 1 or 2 disulfide bonds. All fragmentation methods perform well in the open region, while AI-ETD shows significant increase in coverage for the region enclosed by disulfide bonds.
