Figure 1.

Activation and expansion of Tconv and Tregs in allogenic lymphoreplete hosts. (A,B) 20–40 × 10⁶ B6 splenocytes (CD45.2) were transferred into syngenic B6 CD45.1 vs. allogenic B6xNOD (CD45.2/CD45.1) mice. Five hours post-transfer, mice were sacrificed and their splenocytes analyzed by FACS using AF700 anti-CD45.2, PE-Cy7 anti-CD45.1, FITC anti-TCRβ, e450 anti-CD4, PE anti-Nur77, and APC anti-Foxp3 mAb. (A) Representative dot plots gated on either Foxp3⁻ (Tconv) or Foxp3⁺ (Tregs) live donor CD45.1⁻CD45.2⁺TCRβ⁺CD4⁺ cells and (B) percentages of Nur77⁺ cells in donor T cells recovered from indicated host are shown. (C,D) 20–30 × 10⁶ VPD450-labeled Foxp3-EGFP splenocytes (CD45.2) were transferred into B6 CD45.1 vs. B6xNOD mice. Three days post-transfer, mice were sacrificed and their splenocytes analyzed by FACS using PE anti-CD45.2, APC anti-CD45.1, PE-Cy7 anti-TCRβ and V500 anti-CD4 mAb. (C) Representative dot plots gated on either Foxp3EGFP⁻ or Foxp3EGFP⁺ live donor CD45.1⁻CD45.2⁺TCRβ⁺CD4⁺ cells and (D) percentages of divided donor T cells in individual mice are shown. Data are pooled from 3 to 4 independent experiments and represented as mean ± standard error of the mean (SEM). NS, non-significant; ***p < 0.001.