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. 2019 Mar 19;10:521. doi: 10.3389/fimmu.2019.00521

Figure 2.

Figure 2

Expansion of both Tconv and Tregs in allogenic lymphopenic hosts. 1 × 105 B6-Tconv or Tregs were cell-sorted from B6-Foxp3EGFP mice and transferred into B6-RAG vs. NSG mice (A,B,D,E). 1 × 105 NOD-Tregs were cell-sorted from NOD-Foxp3EGFP mice and transferred into NSG vs. B6-RAGγc mice (C,F). Mice were sacrificed at day 7 and their splenocytes analyzed by FACS using PE anti-CD45.2, APC anti-CD45.1, PE-Cy7 anti-TCRβ and V500 anti-CD4 mAb. (A) Representative dot plots gated on live cells and (B,C) absolute numbers of recovered CD4+TCRβ+Foxp3EGFP− Treg and/or Foxp3EGFP+ Tconv in individual mice are shown. (D) Representative dot plots and (E,F) percentages of Foxp3EGFP expressing cells gated on CD4+TCRβ+ live cells. Data are pooled from 3 to 4 (A,B,D,E) or 1 (C,F) independent experiments and represented as mean ± standard error of the mean (SEM). NS, non-significant; **p < 0.01, ***p < 0.001.