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. 2019 Mar 19;10:521. doi: 10.3389/fimmu.2019.00521

Figure 3.

Figure 3

Frequencies of alloreactive Tconv and Tregs. Indicated numbers (30–1000) of cell-sorted Tregs or Tconv were transferred into NSG mice. At day 7, mice were sacrificed and their splenocytes analyzed by FACS using APC anti-TCRβ and PE anti-CD4 mAb. (A,C) Absolute numbers of recovered live CD4+TCRβ+Foxp3+ Tregs and CD4+TCRβ+Foxp3 Tconv in individual mice are shown. (B) Percentage of Foxp3EGFP+ cells into individual mice injected with 100 or 300 Tregs that exhibit CD4+TCRβ+ events. (D) Numbers of injected T cells are plotted against the log frequency of negative mice. Mean ± standard error of the mean (SEM) is shown. Data are pooled from 4 independent experiments.