Figure 4.

Adoptive transfer survival of Tconv and Tregs in lymphopenic hosts. (A,B) 5 × 10⁴ Tregs (CD45.1) were mixed with 5 × 10⁴ Tconv (CD45.2) and transferred into male NSG or B6-RAG mice. One day later, mice were sacrificed and their splenocytes analyzed by FACS using PE anti-CD4, APC anti-TCRβ, e450 anti-CD45.1 and AF700 anti-CD45.2 mAb. (A) Representative dot plots gated on live donor CD4⁺ TCRβ⁺ cells. (B) Histogram shows Tregs retrieval as a percentage of Tconv. Mean ± standard error of the mean (SEM) is shown. (C,D) Indicated numbers (3–1000) of CD4⁺ RAG2^−/− Marilyn T cells were transferred into male or female B6-RAG mice. At day 7, mice were sacrificed and their splenocytes analyzed by FACS using APC anti-TCRβ and PE anti-CD4 mAb. (C) Absolute numbers of recovered live CD4⁺TCRβ⁺ Marylin T cells in individual mice are shown. (D) Numbers of injected Marilyn T cells are plotted against the log frequency of negative mice. Data are pooled from 2 (A,B) or 4 (C,D) independent experiments.