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SEC analysis of Grb2 SH2 monomer and dimer. (A) Elution profiles of the monomer (solid line) and the dimer (broken line). (B) Elution profiles of the monomer (solid line) and the dimer (broken line) after heating up to 50°C. The eluates corresponding to monomer, dimer, and oligomer are observed at total volumes of 10.1, 9.4, and 8.7 mL, respectively.
