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SPR analysis of Grb2 SH2 monomer and dimer. (A) Typical sensorgrams for binding of the monomer (400, 800, 1600, 3200, and 6400 nM) to immobilized CD28. (B) Typical sensorgrams for binding of the dimer (400, 800, 1600, 3200, and 6400 nM) to immobilized CD28. (C) Scatchard plots for binding of the monomer (closed circle) and the dimer (open circle).
