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. 2019 Mar 14;15(3):e1007650. doi: 10.1371/journal.ppat.1007650

Fig 1. VZV infection of human monocytes, B cells, NK cells, NKT cells, CD4+ T cells and CD8+ T cells.

Fig 1

(A) Human PBMCs were co-cultured with uninfected- (UI) or VZV-infected HFLs for 48 h then harvested and analyzed using flow cytometry. Specific cell types expressed the following markers: monocytes = CD3-CD56-CD19-CD14hiMHC-II+; B cells = CD3-CD56-CD19+; NK cells = CD3-CD56+; NKT cells = CD3+CD56+; CD4+ T cells = CD3+CD4+CD8-, and; CD8+ T cells = CD3+CD8+CD4-. Black histograms represent isotype control (Iso) stained VZV-infected cells, grey histograms represent UI cells, red histograms represent VZV-Ellen-infected cells (VZV) and blue histograms represent vOka-infected cells (vOka). Numbers on right side of each graph represent % VZV glycoprotein E-positive (gE+) cells. (B-C) Summary of flow cytometry analyses above from VZV-Ellen (B) and vOka (C) infections from 12 and 5 different healthy individuals, respectively, with bar graphs representing average % VZV-gE+ immune cells ± SEM. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; # above monocytes represents P<0.01 (B) and P<0.0005 (C) for significant increases in % VZV-gE+ monocytes compared to all other immune cell populations analyzed. Statistical significance was determined using RM one-way ANOVA with the Greenhouse-Geisser correction and Tukey posttest.