KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit Polyclonal anti-Hsf1 | Gross Lab | N/A |
| Rabbit Polyclonal anti-Rpb1 | Gross Lab | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| cOmplete, EDTA-free Protease Inhibitor Cocktail | Sigma Aldrich | Cat#11873580001 |
| Formaldehyde | Fisher Scientific | Cat#F79-500 |
| Power SYBR Green PCR Master Mix | Fisher Scientific | Cat#4367660 |
| Protein A Sepharose Beads | GE Life Sciences | Cat#17096303 |
| Proteinase K | Sigma Aldrich | Cat#P2308-100G |
| Quick T4 DNA Ligase | New England BioLabs | Cat#M2200L |
| Rapamycin | LC Laboratories | Cat#R5000 |
| RNase DNase Free | Sigma Aldrich | Cat#11579681001 |
| Taqα I | New England BioLabs | Cat#R0149 |
| Critical Commercial Assays | ||
| High-Capacity cDNA Reverse Transcription Kit | Applied Biosystems | Cat#4368814 |
| NEBNext Ultra DNA Library Prep Kit for Illumina | New England BioLabs | Cat#E7370 |
| NEBNext Ultra RNA Library Prep Kit | New England BioLabs | Cat#E7530S |
| RNeasy MinElute Cleanup Kit | QIAGEN | Cat#74204 |
| Deposited Data | ||
| Hsf1 ChIP-seq | (Pincus et al., 2018) | GEO# GSE117653 |
| Hsf1-AA RNA-seq | This paper | GEO# GSE122666 |
| Experimental Models: Organisms/Strains | ||
| Saccharomyces cerevisiae | This paper | N/A |
| Strain background: BY4741 and W303 | ||
| Yeast strains, see Table S2 | ||
| Oligonucleotides | ||
| Primers for 3C analysis, see Table S3 | This paper | N/A |
| Primers for ChIP analysis, see Table S3 | This paper | N/A |
| Primers for strain construction, see Table S3 | This paper | N/A |
| Primers for RT-qPCR, see Table S3 | This paper | N/A |
| Recombinant DNA | ||
| Plasmid pSH47 | Euroscarf | P30119 |
| Plasmid pSR14 | S. Gasser Lab | N/A |
| Plasmid pUG6 | Euroscarf | P30114 |
| Plasmid pUG73 | Euroscarf | P30118 |
| Software and Algorithms | ||
| Bowtie2 | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml | N/A |
| DESeq2 | http://bioconductor.org/packages/release/bioc/html/DESeq2.html | N/A |
| HTSeq-Count | https://www-huber.embl.de/HTSeq | N/A |
| ImageJ 1.48v | https://imagej.nih.gov/ij/ | N/A |
| MACS2 | https://pypi.org/pypi/MACS2/ | N/A |
| Slidebook Software, v4 and v6 | https://www.intelligent-imaging.com/slidebook | N/A |
| Tophat | http://ccb.jhu.edu/software/tophat/index.shtml | N/A |
| Other | ||
| Bioptechs Objective Heater System | Bioptechs | Cat#150803 and Cat#150819-19 |
| Bioruptor Plus | Diagenode | Cat#B01020001 |