Antibodies |
|
|
Rabbit Polyclonal anti-Hsf1 |
Gross Lab |
N/A |
Rabbit Polyclonal anti-Rpb1 |
Gross Lab |
N/A |
Chemicals, Peptides, and Recombinant Proteins |
|
|
cOmplete, EDTA-free Protease Inhibitor Cocktail |
Sigma Aldrich |
Cat#11873580001 |
Formaldehyde |
Fisher Scientific |
Cat#F79-500 |
Power SYBR Green PCR Master Mix |
Fisher Scientific |
Cat#4367660 |
Protein A Sepharose Beads |
GE Life Sciences |
Cat#17096303 |
Proteinase K |
Sigma Aldrich |
Cat#P2308-100G |
Quick T4 DNA Ligase |
New England BioLabs |
Cat#M2200L |
Rapamycin |
LC Laboratories |
Cat#R5000 |
RNase DNase Free |
Sigma Aldrich |
Cat#11579681001 |
Taqα I |
New England BioLabs |
Cat#R0149 |
Critical Commercial Assays |
|
|
High-Capacity cDNA Reverse Transcription Kit |
Applied Biosystems |
Cat#4368814 |
NEBNext Ultra DNA Library Prep Kit for Illumina |
New England BioLabs |
Cat#E7370 |
NEBNext Ultra RNA Library Prep Kit |
New England BioLabs |
Cat#E7530S |
RNeasy MinElute Cleanup Kit |
QIAGEN |
Cat#74204 |
Deposited Data |
|
|
Hsf1 ChIP-seq |
(Pincus et al., 2018) |
GEO# GSE117653
|
Hsf1-AA RNA-seq |
This paper |
GEO# GSE122666
|
Experimental Models: Organisms/Strains |
|
|
Saccharomyces cerevisiae |
This paper |
N/A |
Strain background: BY4741 and W303 |
Yeast strains, see Table S2
|
Oligonucleotides |
|
|
Primers for 3C analysis, see Table S3
|
This paper |
N/A |
Primers for ChIP analysis, see Table S3
|
This paper |
N/A |
Primers for strain construction, see Table S3
|
This paper |
N/A |
Primers for RT-qPCR, see Table S3
|
This paper |
N/A |
Recombinant DNA |
|
|
Plasmid pSH47 |
Euroscarf |
P30119 |
Plasmid pSR14 |
S. Gasser Lab |
N/A |
Plasmid pUG6 |
Euroscarf |
P30114 |
Plasmid pUG73 |
Euroscarf |
P30118 |
Software and Algorithms |
|
|
Bowtie2 |
http://bowtie-bio.sourceforge.net/bowtie2/index.shtml |
N/A |
DESeq2 |
http://bioconductor.org/packages/release/bioc/html/DESeq2.html |
N/A |
HTSeq-Count |
https://www-huber.embl.de/HTSeq |
N/A |
ImageJ 1.48v |
https://imagej.nih.gov/ij/ |
N/A |
MACS2 |
https://pypi.org/pypi/MACS2/ |
N/A |
Slidebook Software, v4 and v6 |
https://www.intelligent-imaging.com/slidebook |
N/A |
Tophat |
http://ccb.jhu.edu/software/tophat/index.shtml |
N/A |
Other |
|
|
Bioptechs Objective Heater System |
Bioptechs |
Cat#150803 and Cat#150819-19 |
Bioruptor Plus |
Diagenode |
Cat#B01020001 |