Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) |
cebpa | NA | Ensembl: ENSG00000245848 |
|
| Strain, strain background (Mus musculus) |
Pou5f1GFP transgenic mouse |
Boiani et al., 2002 | NA | Strain: C57Bl /6 × DBA/2 |
| Strain, strain background (Mus musculus) |
Gt(ROSA)26Sortm1 (rtTA*M2)Jae Col1a1tm3 (tetO-Pou5f1,-Sox2,- Klf4,-Myc)Jae/J |
The Jackson Laboratory | Cat# 011004; RRID:IMSR_JAX:011004 |
Strain: (C57BL/6 × 129S4/ SvJae)F1 |
| Strain, strain background (Mus musculus) |
Pou5f1-GFP OSKM-reprogrammable |
Jaitin et al. (2014), Di Stefano et al. (2016) |
NA | Strain: C57BL /6 × 129 |
| Cell line (Homo sapiens) |
PlatE retroviral packaging cell line |
Cell Biolabs | Cat# RV-101; RRID: CVCL_B488 |
|
| Cell line (Mus musculus) |
S17 stromal cell line | From Dr. Dorshkind, UCLA. (Collins and Dorshkind, 1987) |
RRID: CVCL_E226 | |
| Cell line (Mus musculus) |
Mouse Embryonic Fibroblasts, Irradiated |
GIBCO | Cat# A34180 | |
| Recombinant DNA reagent |
pMSCV-Cebpa-IRES-hCD4 | Produced in-house, (Bussmann et al., 2009) |
NA | |
| Antibody | Mouse monoclonal APC Anti-human CD4 (RPA-T4) |
BD Biosciences | Cat# 555349; RRID: AB_398593 |
Dilution used = 1:400 |
| Antibody | Mouse monoclonal biotin anti-human CD4 (RPA-T4) |
eBioscience | Cat# 13–0049; RRID:AB_466337 |
Dilution used = 1:400 |
| Antibody | Rat monoclonal Anti-Mouse CD16/CD32 (Mouse BD Fc Block) |
BD Biosciences | Cat# 553142; RRID: AB_394654 |
Dilution used = 1:400 |
| Antibody | Rat monoclonal Pe-cy7 Anti-mouse CD19 (1D3) |
BD Biosciences | Cat# 552854; RRID:AB_394495 |
Dilution used = 1:400 |
| Antibody | Mouse monoclonal APC Anti-mouse CD11b (44) |
BD Biosciences | Cat# 561015; RRID:AB_10561676 |
Dilution used = 1:400 |
| Antibody | Rat monoclonal biotin Anti-mouse CD19 (1D3) |
BD Biosciences | Cat# 553784; RRID: AB_395048 |
Dilution used = 1:400 |
| Antibody | Rabbit monoclonal [Y69] to c-Myc |
Abcam | Cat# ab32072; RRID:AB_731658 |
Dilution used = 1:76 |
| Antibody | Goat Polyclonal Anti-Rabbit IgG H and L Alexa Fluor 647 |
Life technologies | Cat# A32733; RRID:AB_2633282 |
Dilution used = 1:2000 |
| Strain, strain background (Escherichia coli) |
E. coli: BL21(DE3) Competent |
New England Biolabs | Cat# C2527I | |
| Peptide, recombinant protein |
Recombinant murine IL-7 |
Peprotech | Cat# 217–17 | |
| Peptide, recombinant protein |
Recombinant murine IL-4 |
Peprotech | Cat# 214–14 | |
| Peptide, recombinant protein |
Recombinant murine IL-15 |
Peprotech | Cat# 210–15 | |
| Peptide, recombinant protein |
ESGRO Recombinant mouse LIF protein |
Merk Millipore | Cat# ESG1106 | |
| Commercial assay or kit |
Click-IT EdU Cytometry assay kit |
Invitrogen | Cat# C10425 | |
| Commercial assay or kit |
miRNeasy mini kit | Qiagen | Cat# 217004 | |
| Commercial assay or kit |
SYBR Green QPCR Master Mix |
Applied Biosystems | Cat# 4309155 | |
| Commercial assay or kit |
Alkaline Phosphatase Staining Kit II |
Stemgent | Cat# 00–0055 | |
| Commercial assay or kit |
High Capacity RNA-to-cDNA kit |
Applied Biosystems | Cat# 4387406 | |
| Chemical compound, drug |
17β-estradiol | Merck Millipore | Cat# 3301 | |
| Chemical compound, drug |
MEK inhibitor (PD0325901) |
Selleckchem | Cat# S1036 | |
| Chemical compound, drug |
Doxycycline hyclate | Sigma-Aldrich | Cat# D9891 | |
| Chemical compound, drug |
L-Ascorbic Acid | Sigma-Aldrich | Cat# A92902 | |
| Chemical compound, drug |
GSK3b inhibitor (CHIR-99021) |
Selleckchem | Cat# S1263 | |
| Other | DMEM Medium | Gibco | Cat# 12491015 | |
| Other | RPMI 1640 Medium | Gibco | Cat# 12633012 | |
| Other | Knockout-DMEM | Gibco | Cat# 10829018 | |
| Other | Neurobasal Medium | Gibco | Cat# 21103049 | |
| Other | DMEM-F12 Medium | Gibco | Cat# 12634010 | |
| Other | Fetal Bovine Serum, E.U.-approved, South America origin |
Gibco | Cat# 10270–106 | |
| Other | Embryonic stem-cell FBS, qualified, US origin |
Gibco | Cat# 10270–106 | |
| Other | KnockOut Serum Replacement |
Gibco | Cat# A3181502 | |
| Other | Pen Strep | Gibco | Cat# 15140122 | |
| Other | L-Glutamine (200 mM) | Gibco | Cat# 25030081 | |
| Other | Sodium Pyruvate (100 mM) | Gibco | Cat# 11360070 | |
| Other | MEM Non-Essential Amino Acids Solution (100X) |
Gibco | Cat# 11140068 | |
| Other | 2-Mercaptoethanol | Invitrogen | Cat# 31350010 | |
| Other | N-2 Supplement (100X) | Gibco | Cat# 17502048 | |
| Other | B-27 Serum-Free Supplement (50X) |
Gibco | Cat# 17504044 | |
| Other | TrypLE Express Enzyme (1X) |
Gibco | Cat# 12605010 | |
| Other | Trypsin-EDTA (0.05%) | Gibco | Cat# 25300054 | |
| Other | MACS Streptavidin MicroBeads |
Miltenyi Biotec | Cat# 130-048-101 | |
| Other | MACS LS magnetic columns |
Miltenyi Biotec | Cat# 130-042-401 | |
| Software, algorithm | R | R Project for Statistical Computing http://www.r-project.org/ |
RRID:SCR_001905 |