Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Gene (Homo sapiens)	Werner Syndrome RecQ Like Helicase (WRN)	N/A	Entrez Gene: 7486
Genetic reagent (Homo sapiens)	NTC siRNA	Dharmacon	D-001810–10	non-targeting siRNA pool
Genetic reagent (Homo sapiens)	WRN siRNA pool	Dharmacon	L-010378–00	WRN-targeting siRNA pool
Genetic reagent (Homo sapiens)	WRN siRNA	Dharmacon	J-010378–05	WRN-targeting siRNA
Cell line (Homo sapiens)	HCT 116	ATCC	RRID:CVCL_0291	MSI-H CRC cell line
Cell line (Homo sapiens)	RKO	ATCC	RRID:CVCL_0504	MSI-H CRC cell line
Cell line (Homo sapiens)	SW480	ATCC	RRID:CVCL_0546	MSS CRC cell line
Cell line (Homo sapiens)	SK-CO-1	ATCC	RRID:CVCL_0626	MSS CRC cell line
Cell line (Homo sapiens)	hTERT RPE-1	ATCC	RRID:CVCL_4388	Non-transformed telomerase immortalized cell line
Antibody	mouse anti-WRN	Cell Signaling	RRID:AB_10692114	1/1000 dilution for immunoblot
Antibody	mouse anti-GAPDH	Abcam	RRID:AB_2107448	1/30000 dilution for immunoblot
Antibody	mouse anti-FLAG	SIGMA	RRID:AB_262044	1/1000 dilution for immunoblot
Antibody	mouse anti-LAP2ß	BD Transduction Laboratories	RRID:AB_398313	1/100 for immunofluorescence analysis
Recombinant DNA reagent	pLVX-WRN-3x FLAG-IRES-Puro	This study		Lentiviral vector for stable expression of WRN wild-type
Recombinant DNA reagent	pLVX-WRN-3x FLAG-IRES-Puro E84A	This study		Lentiviral vector for stable expression of WRN E84A mutant
Recombinant DNA reagent	pLVX-WRN-3x FLAG-IRES-Puro K577M	This study		Lentiviral vector for stable expression of WRN K577M mutant
Recombinant DNA reagent	pLVX-WRN-3x FLAG-IRES-Puro E84A _K577M	This study		Lentiviral vector for stable expression of WRN E84A/K577M double mutant
Other	Drive database	PMID 28753431		Functional genomics database on cancer cell line dependencies