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. 2019 Mar 26;8:e43204. doi: 10.7554/eLife.43204

Figure 2. Interactions of the A12.2C with the A135 External domain one and Hybrid binding domain.

Figure 2.

(A) Two interfaces are differently arranged in Pol I* versus Pol I. Both A34.5-Ct and A12.2C can bind to the A135 External Domain 1 (ED1, red), and A34.5-Ct and the N-terminal tail of A135 (A135-Nt) can bind to the A135 Hybrid Binding domain (HB). (B). In the ED1, the A12.2C interacts with both A135 N683 and N684 through Y96 and T98, respectively. (C). In the ED1, the A34.5-Ct interacts with A135 N683 and N684 through R154 and Y150, respectively. (D) In the HB surface, the A135-Nt folds back and positions R12 next to D990. (E) A34.5-Ct interacts with D990 from the HB domain through R157. Densities shown for panels B-E are from the sharpened Pol I* and Pol I EC (+GMPCPP). See also Figure 1—figure supplement 2.