Key resources table.

Designation	Source or reference	Identifiers	Additional information
mrva	NA	FlyBase:FBgn0035763
qsox1	NA	FlyBase: FBgn0033814
C1GalTA	NA	FlyBase: FBgn0032078
srp-Gal4	PMID: 15239955
srp-3xmCherry	PMID: 29321168	RRID:BDSC_78358 and 78359
srp-H2A::3xmCherry	PMID: 29321168	RRID:BDSC_78360 and 78361
UAS-CG8602::FLAG::HA	PMID: 22036573
mrva3102	Bloomington Drosophila Stock Center (BDSC), RRID:SCR_006457	RRID:BDSC_17262
Df(3L)BSC117	BDSC, RRID:SCR_006457	RRID:BDSC_8976
UAS-mCherry.NLS	BDSC, RRID:SCR_006457	RRID:BDSC_38425
C1GalTA2.1	BDSC, RRID:SCR_006457	RRID:BDSC_28834
C1GalTA RNAi 1	Vienna Drosophila Resource Centre (VDRC), RRID:SCR_013805	VDRC: 2826
C1GalTA RNAi 2	VDRC, RRID:SCR_013805	VDRC: 110406
CG8602 RNAi	VDRC, RRID:SCR_013805	VDRC: 101575
qsox1RNAi	VDRC, RRID:SCR_013805	VDRC: 108288
qsox1 KG04615	BDSC, RRID:SCR_006457	RRID:BDSC_13824
MC-38	Other		Gift from Borsig lab, Univ of Zurich (UZH)
4T1	Other	ATCC Cat# CRL-2539, RRID:CVCL_0125	Gift from Borsig lab, UZH
LLC1	Other	ATCC Cat# CRL-1642, RRID:CVCL_4358	Gift from Borsig lab, UZH
B16-BL6	Other	NCI-DTP Cat# B16BL-6, RRID:CVCL_0157	Gift from Borsig lab, UZH
S2R+	Other		Gift from Frederico Mauri of the Knoblich lab at IMBA, Vienna
srpHemo-CG8602::3xmCherry	this paper		CG8602 amplified from genome cloned into DSPL172 (PMID: 29321168)
MT-CG8602::FLAG::HA	Drosophila Genomic Resource Center (DGRC), RRID:SCR_002845	DGRC: FMO06045
MT-Qsox1::FLAG::HA	DGRC, RRID:SCR_002845	DGRC: FMO06379
PTS1-GFP	Other		Gift from Dr. McNew
MFSD1-eGFP	this paper		MFSD1 amplified from dendritic cell cDNA library, inserted into Doxycycline inducible expression vector pInducer20
Anti-GFP clone 2B6	Other		Gift from Ogris lab, MFPL Vienna; (1:100) for WB
Anti-GFP clone 5G4	Other		Gift from Ogris lab, MFPL Vienna; (1:50) for immunochemistry
Anti-T-antigen (mouse monoclonal)	PMID: 23584533		(1:5 for immunochemistry; 1:10 for WB)
Anti-profilin (mouse monoclonal)	Developmental Studies Hybridoma Bank (DSHB), RRID:SCR_013527	DSHB Cat# chi 1J, RRID:AB_528439	(1:50)
Anti-GAPDH (rabbit monoclonal)	Abcam, RRID:SCR_012931	Abcam Cat# ab181603, RRID:AB_2687666	(1:10000) for WB
Anti-GRASP65 (rabbit polyclonal)	Thermo Fisher Scientific, RRID:SCR_008452	ThermoFischer Cat# PA3-910, RRID:AB_2113207	(1:200) for immunochemistry
Anti-Rab5 (rabbit monoclonal)	Cell Signaling Technology (CST), RRID:SCR_004431, Clone C8B1	CST Cat# 3547, RRID:AB_2300649	(1:200) for immunochemistry
Anti-Rab7 (rabbit monoclonal)	CST, RRID:SCR_004431, Clone D95F2	CST Cat# 9367, RRID:AB_1904103	(1:200) for immunochemistry
Anti-LAMP1 (rabbit polyclonal)	Abcam, RRID:SCR_012931	Abcam Cat# ab24170, RRID:AB_775978	(1:200) for immunochemistry
Anti- Cnx99a (mouse monoclonal)	DSHB, RRID:SCR_013527	DSHB Cat# Cnx99A 6-2-1, RRID:AB_2722011	(1:5)
Anti- Hrs 27.4 (mouse monoclonal)	DSHB, RRID:SCR_013527	DSHB Cat# Hrs 27–4, RRID:AB_2618261	(1:5)
Anti- Golgin 84 (mouse monoclonal)	DSHB, RRID:SCR_013527	DSHB Cat# Golgin8412–1, RRID:AB_2722113	(1:5)
Anti Rab7 (mouse monoclonal)	DSHB, RRID:SCR_013527	DSHB Cat# Rab7, RRID:AB_2722471	(1:5)
Anti-GMAP (goat polyclonal)	DSHB, RRID:SCR_013527	DSHB Cat# GMAP, RRID:AB_2618259	(1:50)
Anti- Golgin 245 (goat polyclonal)	DSHB, RRID:SCR_013527	DSHB Cat# Golgin245, RRID:AB_2618260	(1:50)
Anti- HA (rat monoclonal)	Roche, RRID:SCR_001326	Roche Cat# 3F10, RRID:AB_2314622	(1:50)
Anti-LanA (rabbit polyclonal)	PMID:9257722		gift from Stefan Baumgartner (1:500)
Anti-Vasa (rat monoclonal)	DSHB, RRID:SCR_013527	DSHB Cat# anti-vasa, RRID:AB_760351	(1:25)
Alexa 488- or 557- or 633- secondaries	Thermo Fisher Scientific, RRID:SCR_008452		(1:500 for 488 and 557; 1:100 for 633)
Goat-anti-rabbit IgG (H + L)-HRP	BioRad	Bio-Rad Cat# 170–6515, RRID:AB_2617112	(1:10000)
Goat-anti-mouse IgG (H/L):HRP	BioRad	Bio-Rad Cat# 170–6516, RRID:AB_11125547	(1:10000)
LysoTracker Green DND-26	Invitrogen, RRID:SCR_008410	L7526	75 nM
Alexa Fluor 488 Phalloidin	Invitrogen, RRID:SCR_008410	A12379	(1:500)
Vectashield mounting medium	Vector Laboratories, RRID:SCR_000821	VectorLabs: H-1000
Vectashield mounting medium with DAPI	Vector Laboratories, RRID:SCR_000821	VectorLabs: H-1200
Halocarbon Oil 27	Sigma-Aldrich, RRID:SCR_008988	Sigma Aldrich: Cat# H8773
srpHemo-mrva	this paper		CG8602 amplified from genome cloned into srpHemo plasmid
srpHemo-MFSD1	this paper		mmMFSD1 amplified from dendritic cell cDNA library cloned into srpHemo plasmid
Mrva fw	Fly Primer Bank		qPCR; 5'TGTGCTTCGTGGGAGGTTTC
Mrva rv	Fly Primer Bank		qPCR; 5'GCAGGCAAAGATCAACTGACC
C1GalTA fw	Fly Primer Bank		qPCR; 5'TGCCAACAGTCTGCTAGGAAG
C1GalTA rv	Fly Primer Bank		qPCR: 5'CTGTGATGTGCATCGTTCACG
Ugalt fw	Fly Primer Bank		qPCR; 5'GCAAGGATGCCCAGAAGTTTG
Ugalt rv	Fly Primer Bank		qPCR; 5'GATATAGACCAGCGAGGGGAC
RpL32 fw	Fly Primer Bank		qPCR; 5'AGCATACAGGCCCAAGATCG
RpL32 rv	Fly Primer Bank		qPCR; 5'TGTTGTCGATACCCTTGGGC
Lectin staining kit #2	EY Laboratories	EYLabs:FLK-002
FIJI	http://fiji.sc/ RRID:SCR_002285)
Imaris	http://www.bitplane.com/imaris/imaris, RRID:SCR_007370
Matlab	https://www.mathworks.com/products/matlab.html, RRID:SCR_001622
FlowJo	https://www.flowjo.com/RRID:SCR_008520
LaVision ImSpector	http://www.lavisionbiotec.com/, RRID:SCR_015249
Proteome Discoverer 1.4	https://www.thermofisher.com/order/ catalog/product/OPTON-30795, RRID:SCR_014477
LightCycler 480 software	https://lifescience.roche.com/en_at/products/lightcycler14301-480 -software-version-15.html
GraphPad Prism	https://www.graphpad.com/scientific-software/prism/ RRID:SCR_002798