Figure 6. Delayed RHO regeneration in RPEΔAbca1;Abcg1 mice.
Dark-adapted 2-months-old Ctr and RPEΔAbca1;Abcg1 mice were exposed to 5’000 lux for 10 min and the RHO content was measured in each retina. Dark controls were kept in darkness for the entire procedure. RHO levels were measured in dark controls, immediately after bleach (0 min) and after 30, 60 and 120 min of recovery in darkness. (A) ‘Total’ amount of regenerated RHO after 120 min was calculated by subtracting the corresponding averaged RHO amount at ‘0 min’ from the RHO levels at ‘120 min’. (B) Amount of regenerated RHO during the indicated time intervals after bleaching were calculated by subtracting the corresponding averaged RHO amount at the early time point from the RHO levels measured at the later time point. Shown are data from individual samples and means ± SD (N = 4–8 eyes, corresponding to 2–4 mice). Statistics: Student’s t-test vs ‘Ctr’; *: p<0.05. Averages and SD of RHO content measurements can be found in Supplementary file 1B; single measurements per eye can be found in Figure 6—source data 1.