Figure 7. Age-dependent retinal degeneration in RPEΔAbca1;Abcg1 mice.
(A) Fundus images (upper panels) and OCT scans (lower panels, corresponding to red lines in fundus) of Ctr and RPEΔAbca1;Abcg1 mice at the indicated age. White arrowheads indicate sub-retinal hyper-reflective foci. Retinal morphology of the same animals was analyzed by light microscopy (B). Representative pictures of N ≥ 3 animals per group. ONL thickness was quantified from nasal-temporal panorama images at 2 and 6 months of age and presented as spidergrams (C): significant ONL thinning was detected in 6-months-old RPEΔAbca1;Abcg1 mice. Shown are means ± SD (N ≥ 3). Statistics: two-way ANOVA with Sidak’s multiple comparison test; *: p<0.05, **: p<0.01, ****: p<0.0001. Abbreviations as in Figure 1.
Figure 7—figure supplement 1. Absence of a phenotype in the inner retina of RPEΔAbca1;Abcg1 mice at 6 months of age.
(A) INL thickness was quantified from nasal-temporal panorama images at 6 months of age and presented as spidergram. Shown are means ± SD (N ≥ 3). Statistics: two-way ANOVA with Sidak’s multiple comparison test. (B) Retinal sections from 6-months-old Ctr (upper panel) and RPEΔAbca1;Abcg1 (lower panel) mice were stained for POU4F1 (red); nuclei were counterstained with DAPI. Note that the used secondary antibody was an anti-mouse and stained retinal blood vessels as well. Representative images of N = 3 animals per group. GCL: ganglion cell layer.