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. 2017 Aug 20;27(6):345–362. doi: 10.1089/ars.2016.6758

FIG. 4.

FIG. 4.

AOPPs aggravate renal fibrosis in ADR nephropathy. (A) Representative micrographs show that AOPP-accelerated renal interstitial fibrosis. Kidney sections were subjected to PAS and Masson's trichrome staining. Arrow indicates positive staining. Scale bar, 50 μm. (B) Graphical representation of kidney fibrotic lesions in different groups after quantitative determination. *p < 0.05 versus normal controls; †p < 0.05 versus ADR alone (n = 5–6). ADR/A indicates ADR plus AOPP group. (C–F) Western blot analyses of renal expressions of fibronectin, α-SMA, and MMP-7 in different groups. Numbers (1–3) represent different animals in a given group. Graphical representations of fibronectin (D), α-SMA (E), and MMP-7 (F) expressions in different groups as indicated. *p < 0.05 versus normal controls; †p < 0.05 versus ADR alone (n = 5–6). (G) Representative micrographs show fibronectin, α-SMA, Fsp-1, and collagen I expressions in different groups. Paraffin sections were stained with different antibodies. Boxed areas are enlarged and presented in the right column. Arrows indicate positive staining. Scale bar, 50 μm. All full unedited gels for Figure 4 are presented in Supplementary Figure S8. Fsp-1, fibroblast-specific protein 1; MMP-7, matrix metalloproteinase; α-SMA, α-smooth muscle actin.