Figure 4.

PDT-induced CAF depletion. a) A photograph showing the setup of the PDT experiment. A 671 nm laser was applied to the right-hand-sided tumor at 24 h post scFv-Z@FRT injection. The left-hand-sided tumor was covered by aluminum foil. b) Confocal microscopic images of 4T1 tumor slices stained with FITC-conjugated anti-α-SMA antibody. Positive α-SMA staining was dramatically reduced two days after PDT. Green, FITC (α-SMA); blue, DAPI. Scale bars: 100 μm. c) Mason’s trichrome staining results. Blue, collagen; red, muscle fibers; black/blue, nuclei.