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. Author manuscript; available in PMC: 2020 Mar 26.
Published in final edited form as: Biomater Sci. 2019 Mar 26;7(4):1345–1357. doi: 10.1039/c8bm01556b

Figure 2.

Figure 2

Characterizations of IL-2/Fc NGs. (a) Representative high-performance liquid chromatography (HPLC) analysis of native IL-2/Fc and IL-2/Fc NGs with a size-exclusion column. (b) Representative sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of native IL-2/Fc and IL-2/Fc NGs with or without (w/o) a reducing agent, dithiothreitol (DDT). Black arrow: IL-2/Fc homodimer; Red arrows: IL-2/Fc monomer (disulfide linkage in the hinge region was cleaved by DTT to release monomers). (c) Representative transmission electron microscopy (TEM) images of and IL-2/Fc NGs. (d) Hydrodynamic diameter and polydispersity of IL-2/Fc NGs were determined by dynamic light scattering measurement. (e) Accumulative release of IL-2/Fc from IL-2/Fc NGs in buffers with glutathione (GSH) at different concentrations.