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. Author manuscript; available in PMC: 2020 Mar 25.
Published in final edited form as: Dev Cell. 2019 Jan 31;48(6):853–863.e5. doi: 10.1016/j.devcel.2019.01.001

Figure 2. Vitamin D Signaling Enhances Organismal Size.

Figure 2.

(A) MIP images and quantification of H3P staining of 3 dpf embryos. n = 11-13. Scale bar, 500 μm.

(B) Example and quantification of 4 dpf zebrafish growth. Scale bar, 500 μm. n = 12-14.

(C) Growth and rescue of 2 mpf juvenile WT and ubb:cyp24a1 zebrafish. Scale bar, 4 mm.

(D) Quantification of zebrafish length before and after treatment. n = 14-15.

(E) mGFP and TagBFP expression in 4 dpf embryos treated with vehicle or 10 nM Alfa. Scale bar, 500 μm.

(F) Quantified body length of two independent ubb:cyp24a1 lines and WT siblings at 4 dpf. n=14-23.

(G) cyp24a1mGFP expression in 1 μM Alfa-treated embryos.

(H) Quantification of relative growth of 4 dpf embryos treated with vehicle or Alfa for 24 hours. n = 13.

(I) Representative images and body length quantification of 6 mpf zebrafish. Scale bar, 5 mm. n = 7.

(J) mGFP and TagBFP expression of 3 dpf embryos after a single heat-shock and 24-h drug treatment. Scale bar, 500 μm.

(K) Quantified relative growth of 4 dpf embryos after a heat shock and 24-h drug treatment. n = 10.

See also Figure S2.