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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Vascul Pharmacol. 2019 Feb 27;114:1–12. doi: 10.1016/j.vph.2019.02.004

Figure 2. Timeline for generating CRISPRized mice.

Figure 2.

The continued refinement in CRISPR components (e.g., store-bought ribonucleoprotein complexes [RNP] of Cas9 and guide RNA) as well as methods of delivery (microinjection, MI versus electroporation, EP) allow for the generation of genetically-modified mice in as little as 5 weeks. Critical steps include the strategy in design with PCR primers (P1, P2) for genotyping founder mice by a PCR restriction fragment length polymorphism (RFLP)-like method and testing for offtargets by Sanger sequencing. Note that germline transmission of CRISPR edited genes typically can occur within 3 months from conception of the project.