Bioluminescence of serum and urine collected from mice at 1, 6, and 24 h post i.v. delivery of (A) teLuc-, (B) Antares2-, or (C) Akaluc-expressing HEK293T cells, suggesting that luciferases were released into blood and that the small teLuc luciferase further entered urine. Data are presented as mean and s.d. of three biological replicates. In panel C, results are shown for samples with and without supplementing ATP.