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. Author manuscript; available in PMC: 2020 Mar 26.
Published in final edited form as: Biochemistry. 2019 Mar 11;58(12):1689–1697. doi: 10.1021/acs.biochem.8b01303

Figure 7.

Figure 7.

(AB) Representative fluorescence images of HEK293T cells expressing PercevalHR and either FLuc (A) or teLuc (B), upon addition of D-luciferin (A) or DTZ (B). Iodoacetic acid (IAA) was used to treat cells after D-luciferin to completely deplete ATP. Scale bars: 10 μm. (C) PercevalHR occupancy and estimated ATP:ADP ratio changes upon treatment with D-luciferin or DTZ. Images were captured with 488 nm excitation, and the emission was collected from 510 to 600 nm.