Table 1. Primers used in the qRT-PCR assay.

Gene	Primer sequence	Fragment length	Annealing temperature
Col10a1	F: 5′-GATCATGGAGCTCACGGAAAA-3′	63 bp	60°C
	R: 5′-CCGTTCGATTCCGCATTG-3′
Col2a1	F: 5′-GGTGGAGCAGCAAGAGCAA-3′	83 bp	60°C
	R: 5′-CGTCGCCGTAGCTGAAGTG-3′
Aggrecan	F: 5′-ACTGAAGGACAGGTTCGAGTG-3′	133 bp	60°C
	R: 5′-CACACCGATAGATCCCAGAGT-3′
Pparγ	F: 5′-ACCACGGTTGATTTCTCCAG-3′	242 bp	60°C
	R: 5′-GCTTTATCCCCACAGACTCG-3′
Sox9	F: 5′-GTGCTGAAGGGCTACGACTGGA-3'	159 bp	64°C
	R: 5′-GTTGTGCAGATGCGGGTACTGG-3′
Mmp13	F:5'-GAGTTGGACTCACTGTTGGTC-3'	216 bp	58°C
	R: GCAAGAGTCACAGGATGGTAG-3'
β-actin	F: 5′-GCTGTGTTGTCCCTGTATGC3′	106 bp	56°C
	R: 5′-GAGCGCGTAACCCTCATAGA3′
Rno-miR-27a-3p	RMIRQP0359GeneCopoeia, China	-	60°C
Rno-miR-27b-3p	RMIRQP0361GeneCopoeia, China	-	60°C
Rat-U6	RMIRQP9003GeneCopoeia, China	-	60°C