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(A) Expression of ANKRD49 mRNA was measured by q-PCR in four human glioma cell lines: U251, U87, U373, and A172. (B,C) Quantitative RT-PCR analysis revealed that ANKRD49 expression was efficiently knocked down in the U251 (B) or U87 (C) cells; ***P<0.001. (D) Western blot analysis revealed that ANKRD49 expression was efficiently knocked down in the U251 or U87 cells. GAPDH served as an internal control.
