Figure 6. Alterations of protein modification induced by ANKRD49 knockdown are detected through stress and apoptosis signaling assay and Western blot.

(A) The post-translational modification and total protein level of 18 important signaling proteins (13 proteins for phosphorylation, 2 ptoteins for expression level, and 3 proteins for cleavage) was monitored by array hybridization. α-tubulin is used as an internal control; *P<0.05 and **P<0.01. (B–D) Western blot analysis of shANKRD49 or shCtrl U251 cells with antibodies against survivin, p-ERK, ERK (B), and p-HSP27, HSP27, p-P53, p53 (C), and p-Smad2, Smad2, p-Chk1, Chk1 (D).