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. 2018 Oct 15;38(5):BSR20180768. doi: 10.1042/BSR20180768

Figure 7. Binding pockets and pocket-forming sites residues identification.

Figure 7

(A) MetaPocket 2.0 found four pockets including the hole in the center of the trimer (green mesh) and a pocket on each monomer (gray meshes) of SAYabJ that are potential binding sites of substrates. (B) Three charge–charge interactions on the trimeric interface. (C) Detailed view of the one of the monomer pockets. The residues that can be chlorinated in the monomer pocket are labeled. (D) Ribonuclease activity assay was monitored in chlorinated SAYabJ (▓), chlorinated C103A SAYabJ mutant (□), chlorinated pocket-forming sites mutant (Y16H18K39R101C103K111A) (△), and SAYabJ before chlorination (▼). The data from three scans were averaged.