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. 2017 Dec 15;37(6):BSR20170837. doi: 10.1042/BSR20170837

Figure 4. PTEN was the target gene of miR-500a.

Figure 4

(A) Diagram of the miR-500a putative binding sites and corresponding mutant sites in the 3′-UTR of PTEN. (B) Effects of miR-500a on the expression of PTEN 3′-UTR-containing reporter genes. (C) The protein expression of PTEN, p-Akt (S473), p-Akt (T308), Akt, p-mTOR, mTOR, p-4E-BP1, 4E-BP1, p-S6K, and S6K in SMMC-7721 cells after transfection with mimic miR-500a or inhibitor miR-500a. (D–I) The relative optical density of PTEN, p-Akt (S473)/Akt, p-Akt (T308)/Akt, p-mTOR/mTOR, p-4E-BP1/4E-BP1, and p-S6K/S6K in SMMC-7721 cells after transfection with mimic miR-500a or inhibitor miR-500a. *P<0.05 and **P<0.01, compared with the NC group, the relative optical density in mimic miR-500a or inhibitor miR-500a group had a significant difference.