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. 2019 Mar 20;9:173. doi: 10.3389/fonc.2019.00173

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Copyright © 2019 Smith, Mathew, Gaikwad, Rech, Burney, Faro, Lucci, Bai, Olsen and Byrd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The C-33A (HPV-) and SiHa(HPV 16/18+) human cervical cancer cell lines were supplemented in vitro with a one-time dose of AHCC 0.42 mg/mL and then incubated for 72 h. The study was completed in duplicate as described in materials and methods. While HR-HPV was suppressed for 24 h, it was not cleared evident by expression at 48 and 72 h. NT, non-treatment control. (B) The C-33A (HPV-) and SiHa(HPV16/18+) human cervical cancer cell lines were supplemented in vitro with daily fresh dose of AHCC 0.42 mg/mL once every 24 h for 5 days (120 h) as described in materials and methods. NT, non-treatment control. This was successful to eliminate HR-HPV expression in the SiHa cell line.