Table 2.

The primers used in the current study.

Primer	Sequence (5′ to 3′)	Target gene	Amplicon size (bp)	Reference
VparaF VparaR	GCTGACAAAACAACAATTTATTGTT	16S rRNA	170	Rojas et al., 2011
	GGAGTTTCGAGTTGATGAAC
toxRS/old-F toxRS/old-R	TAATGAGGTAGAAACG	toxRS sequence of theold O3:K6 clone	651	Okura et al., 2003
	ACGTAACGGGCCTACG
toxR-F toxR-R	GTCTTCTGACGCAATCGTTG	toxR	368	Kim et al., 1999
	ATACGAGTGGTTGCTGTCATG
L-tdh R-tdh	GTAAAGGTCTCTGACTTTTGGAC	Thermostable direct hemolysin	269	Nishibuchi and Kaper, 1995
	TGGAATAGAACCTTCATCTTCACC
L-trh R-trh	TTGGCTTCGATATTTTCAGTATCT	TDR-related hemolysin (TRH)	500	Rojas et al., 2011 Silva et al., 2018
	CATAACAAACATATGCCCATTTCCG
VP0950-F VP0950-R	GCCAAACTTCTCAAACAACA	Biofilm	298	Chao et al., 2010
	ATGAAACGCAATTTACCATC
VP0952-F VP0952-R	TATGATGGTGTTTGGTGC		276
	TGTTTTTCTGAGCGTTTC
VP0962-F VP0962-R	GACCAAGACCCAGTGAGA		358
	GGTAAAGCCAGCAAAGTT
VP0634-F VP0634-R	AGATGTCTTTGTTCACCCT	VPaI-2	473	Chao et al., 2010
	CGAAGTCGGCTTTGTAGTT
VP0636-F VP0636-R	TGAAAGTGACGGCTCCAATC	VpaI-2	207
	CTGCGTTCAGTTCCACATCG
VP1094-F VP1094-R	GATTCAAGGTGGATTTCG	VpaI-3	219
	ATAAGCGGGTTCTTCGTC
VP1253-F VP1253-R	GTCCCTCAATCTGTGCTT	VpaI-6	898
	GCTGACAATCTTCGCTCT
VP1263-F VP1263-R	TCGTGGACAACTATGAAGC	VpaI-6	293
	AAGTAGGAACTGACGGAAAC
VP1409-F VP1409-R	TGTTGCTTTCTATTGCGAC	T6SS	869
	CCATAACGACTTTTCTTTC
VP1418-F VP1418-R	AAACCAGCCTCAGCAACAAG	T6SS	308
	TAATAGCGGCGATAAATCCA
VP1510-F VP1510-R	TTCAGGTTTCAGGGTTC	Type I pilus	511
	GCTTGCTCATAGTTGGC
VP1506-F VP1506-R	CCGAACATTTAGAAGGC	Type I pilus	399
	AGCGAGAAAGCAGAACA
VP1677-F VP1677-R	TAGTCAGATAGCAACCAACA	T3SS1	548	Chao et al., 2009
	CATCAGCGAAATGAGAAACA
VP1690-F VP1690-R	CACCAATGTGAGCCAAAAAG	T3SS1	384
	ATAAACACCGATGCCGAAGC