Figure 3. MiR-182-5p is up-regulated, whereas ADCY6 and the Rap1/MAPK signaling pathway are inhibited after treatment with ALN.
(A) miR-182-5p expression and mRNA levels of ADCY6, ERK1, ERK2, Rap1 and P38 MAPK in response to ALN treatment; (B) the gray value of ADCY6, ERK1/2, p-ERK1/2, P38 MAPK, p-P38 MAPK and Rap1 protein bands in response to ALN treatment; (C) protein levels of ADCY6, ERK1/2, Rap1 and P38 MAPK and the extent of ERK1/2 and P38 MAPK phosphorylation in response to ALN treatment; *, P < 0.05 vs. the sham group; #, P < 0.05 vs. the OP group. MiR-182-5p, microRNA-182-5p; OP, osteoporosis; ADCY6, adenylate cyclase 6.