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. 2018 Dec 21;38(6):BSR20180696. doi: 10.1042/BSR20180696

Figure 8. Down-regulated miR-182-5p enhances osteoblast differentiation.

Figure 8

(A and B) ALP activity and BGP activity in response to the treatment of miR-182-5p mimic, miR-182-5p inhibitor, si-ADCY6, miR-182-5p inhibitor + si-ADCY6, U0126 or miR-182-5p inhibitor + U0126; (C) the gray value of ALP, BGP, COL I and BMP2 protein bands in response to the treatment of miR-182-5p mimic, miR-182-5p inhibitor, si-ADCY6, miR-182-5p inhibitor + si-ADCY6, U0126 or miR-182-5p inhibitor + U0126; (D and E) mRNA and protein levels of COL I in response to the treatment of miR-182-5p mimic, miR-182-5p inhibitor, si-ADCY6, miR-182-5p inhibitor + si-ADCY6, U0126 or miR-182-5p inhibitor + U0126; (F and G) mRNA and protein levels of ALP in response to the treatment of miR-182-5p mimic, miR-182-5p inhibitor, si-ADCY6, miR-182-5p inhibitor + si-ADCY6, U0126 or miR-182-5p inhibitor + U0126; (H and I) mRNA and protein levels of BMP2 in response to the treatment of miR-182-5p mimic, miR-182-5p inhibitor, si-ADCY6, miR-182-5p inhibitor + si-ADCY6, U0126 or miR-182-5p inhibitor + U0126. *, P < 0.05 vs. the blank group and the NC group; #, P < 0.05 vs. the miR-182-5p inhibitor group. ALP, alkaline phosphatase; BGP, Bone gla protein; COL I, type I collagen; NC, negative control; miR-182-5p, microRNA-182-5p.