Table 1. The primer sequences for RT-qPCR.

Gene	Sequence
miR-182-5p	F: 5′-GGGTCTAGCTGCCGGAGG-3′
	R: 5′-CGGTGTGAGTTCTACCATTGC-3′
U6	F: 5′-GCTCGCTTCGGCAGCACA-3′
	R: 5′-GAGGTATTCGCACCAGAGGA-3′
ADCY6	F: 5′-CAGCAGGGTAGTGTGTGCAG-3′
	R: 5′-TCTGCATTTGATTTTGGCCT-3′
ERK1/2	F: 5′-CTCAAGCCTTCCAACCTC-3′
	R: 5′-TTCCACGGCACCTTATTT-3′
P38 MAPK	F: 5′-GCATAAAGCCCAAGAGAACCAAC-3′
	R: 5′-GACAGGACAAGCTCCAGAGCACA-3′
Rap1	F: 5′-GCGTCTGGTCA GAGAGCTG-3′
	R: 5′- TCAATCCTCCGAGCTACATTCT-3′
β-actin	F: 5′-GGCACCACACTT TCTACAAT-3′
	R: 5′-AGGTCTCAAACATGATCTGG-3′
PCNA	F: 5′-CGGCGTGAACCTACAGA-3′
	R: 5′-TCGCAGCGGTATGTGTCGAA-3′
BMP2	F: 5′-ACGATGCCGCCATTTGTG-3′
	R: 5′-CGCCTCGCCTTCTTCAGT-3′
BGP	F: 5′-AGCGACTCTGAGTCTGACAAA-3′
	R: 5′-AACGGTGGTGCCATAGATGCG-3′
ALP	F: 5′-CAGTGGTATTGTAGGTGCTGTG-3′
	R: 5′-TTTCTGCTTGAGGTTGAGGTTAC-3′
COL1A1	F: 5′-GTCCTATGGCTATGATGAGAAATC-3′
	R: 5′-CACCATCCAAACCACTGAAAC-3′

Abbreviations: ADCY6, adenylate cyclase 6; ALP, alkaline phosphatase; BGP, bone gla protein; BMP2, bone morphogenetic protein-2; COL I, type I collagen; ERK1/2, extracellular signal-regulated kinase 1/2; F, forward; miR-182-5p, microRNA-182-5p; PCNA, proliferating cell nuclear antigen; R, reverse; Rap1, Ras-related protein 1; RT-qPCR, quantitative real-time polymerase chain reaction.