Table 3. Susceptibility testing of M. smegmatis WT and mutant strains.
| MIC50 (µg/ml)1 | Fold change compared with WT | |||||||
|---|---|---|---|---|---|---|---|---|
| INH | RIF | Orlistat | CyC17 | INH | RIF | Orlistat | CyC17 | |
| WT | 1.4 | 0.76 | 0.97 | 0.56 | - | - | - | - |
| lipGMS::Hyg | 2.9 | 3.3 | 6.5 | 9.6 | ×2.0 | ×4.3 | ×6.7 | ×15.7 |
| lipGMS::Hyg Comp | 1.8 | 0.89 | 1.2 | 0.60 | ×1.2 | ×1.2 | ×1.3 | ×0.94 |
| lipGMS::Hyg CompMTB | 1.9 | 0.96 | 1.6 | 0.65 | ×1.3 | ×1.3 | ×1.6 | ×1.1 |
1
MIC50 values corresponding to the compound minimal concentration leading to 50% growth inhibition have been determined by the REMA method and are expressed as mean values of three independent assays performed in duplicate.