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. 2018 Dec 21;38(6):BSR20180978. doi: 10.1042/BSR20180978

Figure 3. miR-141 regulated MAP4K4 expression.

Figure 3

(A) predicted miR-141 target sequence in the 3′-UTR of MAP4K4. (B) Dual-luciferasereporter assay of the HCT-116 cells transfected with the MAP4K4-3′-UTR reporter, the MAP4K4-3′-UTR-mutation reporter, miR-141 mimics, inhibitor, and mimic NC. Being linked to the segment containing the target sequence within the 3′-UTR in MAP4K4 mRNA, miR-141 expression reduced the luciferase activity. (C) 24 h h after the transfection, cells were harvested for RT-qPCR analysis using miR-141 primers. Overexpression of miR-141 significantly reduced the MAP4K4 mRNA expression levels in CRC cells. (D) MAP4K4 proteins levels in CRC cells treated with miR-141 mimics, inhibitors and NC; n=3, *P<0.05.