Table 1. Primer sequences of SNPs in CACNA 1A, CACNA 1C, and CACNA 1H genes for PCR-RFLP.

Primer	Primer sequence	Annealing temperature	Annealing time	Cycle time
rs2248069	F: 5′-CCGAAAAGACTTCGACTCCG-3′	66°C	45 s	30
	R: 5′-TCCATCCCTGGGCCCCAGGA-3′
rsl6030	F: 5′-ATAGGGAATGTCCACGACGC-3′	58°C	45 s	40
	R: 5′-GTGTGTTCTCACTTATAATG-3′
rs216008	F: 5′-GACAAGTTTTAGCGGTACTT-3′	58°C	45 s	45
	R: 5′-AGGCCTACCTTGGGTTTGAA-3′
rs2239050	F: 5′-TTGATAACTGGGGAGGAACT-3′	56.5°C	45 s	30
	R: 5′-GATGTCCGTTTTCTTCATTT-3′
rs3794619	F: 5′-TTTAACGGTCTCACCGGGAT-3′	58°C	45 s	40
	R: 5′-ATTATCCAGTCTGGAAGAGT-3′
rs7191246	F: 5′-TAGACTCGTTCCGAACAGTCT-3′	52°C	45 s	35
	R: 5′-ACACAAGCATACGGTCCCTC-3′

Abbreviations: F, forward; R, reverse.