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. 2018 Jul 6;38(4):BSR20180704. doi: 10.1042/BSR20180704

Figure 2. Effect of MIAT silencing on the survival of MCF7 cells.

Figure 2

MCF7 cells (n=4 cultures) were transfected with the indicated MIAT siRNA or negative control (NC) siRNA and harvested at 72 h post-transfection. Cells were replated for assessment of cell survival after a further 48 h. (A) At 72 h post-transfection, RT-qPCR analysis confirmed decreased cellular MIAT levels in cells treated with MIAT siRNAs. (B) Cells transfected with MIAT siRNAs expressed less OCT4 mRNA as confirmed with RT-qPCR. (C) Silencing of MIAT enhanced basal apoptosis. (D) Short-term viability was reduced 48 h post replating. (E) Colony formation in long-term clonogenic assays was significantly decreased. (F) Cell cycle analysis revealed that MIAT silencing caused an elevation in the percentage of cells in G0/G1 phase; *P<0.05, **P<0.01, and ***P<0.001 versus cells transfected with NC siRNA (one-way ANOVA and Bonferrroni’s MCT).