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. 2019 Mar 20;10:263. doi: 10.3389/fphar.2019.00263

FIGURE 3.

FIGURE 3

Influence of CysLT1RA on PPARγ target gene expression in 3T3-L1 adipocytes. Semi-quantitative mRNA expression of (A) GLUT-4, (B) adiponectin, (C) FABP-4, and (D) lipoprotein lipase in 3T3-L1 adipocytes differentiated in the presence of 0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin plus the PPARγ agonist rosiglitazone (2 μM) or zafirlukast and montelukast. Results are shown as fold induction of the DMSO treated control (2-ΔΔct method). Results are given as mean + SEM of three to six independent experiments. Western Blot experiments showing (E) CD36 and (F) FABP-4 protein expression in 3T3-L1 adipocytes differentiated in presence of 0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin plus the PPARγ agonist rosiglitazone or zafirlukast or montelukast. DMSO (w/o) treated cells were used as negative control. Densitometry results are presented as mean + SEM. One representative western blot experiment out of three is shown. Significant changes versus the untreated control are indicated with an asterisk. ∗P ≤ 0.05, ∗∗P ≤ 0.01, ∗∗∗P ≤ 0.001.