Figure 4.

Flow cytometry analysis of immune cell infiltration into vaginal tissues. (A) Representative flow cytometry plots of APC anti-Gr-1 vs. FITC anti-CD45. Values in gated regions indicate percentages of singlet non-debris events. (B) Quantification of flow cytometry analysis for CD45⁺ cells and Gr-1⁺ cells in vaginal tissues at 7 dpi. Error bars indicate SEM (n = 5 per group). (C) Quantification of flow cytometry analysis for CD3⁺, CD49b⁺, CD11c⁺, and F4/80⁺ cells in vaginal tissues at 7 dpi. Error bars indicate SEM (n = 5 per group). Asterisks denote significant difference by two-tailed unpaired Student's t-test, *p < 0.05, ns, or unlabeled, not significant.