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. 2019 Mar 20;10:519. doi: 10.3389/fmicb.2019.00519

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Copyright © 2019 Wu, Zhang and Fang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increasing pyruvate concentration in M. robertsii conidia by overexpressing the pyruvate kinase gene MAA_06851. (A) qRT-PCR confirmation of the overexpression of MAA_06851 in the conidia of two transformants (T-06851^OE-1 and T-06851^OE-2). The gene expression levels during conidiation in the two transformants (5 days after inoculation of conidia on PDA) were calculated relative to that of the wild-type (WT) strain, which was set to 1. (B) The pyruvate concentration in the conidia grown at optimal conditions (26°C on PDA for 14 days), and in the heat-treated conidia. Data are expressed as mean ± standard error (SE). Values with different letters are significantly different ( p < 0.05, Tukey’s test in one-way ANOVA). All assays were repeated three times with three replicates per repeat.