Table 4.

Analytical parameters (%) of culture (A.) and PCR-DGGE-S (B.) for six most prevalent OTUs regarding the material and method.

	Ca		CUb		DJCPc		DJCDd		DJCUe
	Sensitivity	Specificity	Sensitivity	Specificity	Sensitivity	Specificity	Sensitivity	Specificity	Sensitivity	Specificity
(A)
Enterococcus faecalis	93	37	91	55	69	85	73	82	56	96
Escherichia coli	90	75	88	87	83	83	78	80	60	98
Klebsiella sp.	89	67	100	80	86	92	100	91	60	98
Proteus mirabilis	85	78	75	83	50	98	25	91	NA	98
Pseudomonas aeruginosa	86	85	50	90	67	96	50	95	33	98
CoNSf	83	78	67	88	88	83	75	87	25	100
Average	88	70	78	80	74	90	67	88	39	98
(B)
Enterococcus faecalis	57	86	59	89	56	91	58	90	71	92
Escherichia coli	63	94	70	95	36	98	41	95	86	92
Klebsiella sp.	22	98	26	100	60	98	50	100	75	96
Proteus mirabilis	52	95	45	95	67	96	17	94	NA	98
Pseudomonas aeruginosa	32	99	36	94	50	98	40	96	50	96
CoNS	36	97	18	98	44	98	46	96	100	84
Average	43	95	43	95	52	97	42	95	64	93

^aC, catheters;

^bCU, catheter urine;

^cDJCP, proximal tip of double-J catheter;

^dDJCD, distal tip of double-J catheter;

^eDJCU, double-J catheter urine;

^fCoNS, Coagulase-negative staphylococci. Table (A) shows sensitivity (%) and specificity (%) of culture (PCR-DGGE-S was a reference method), whereas (B) shows sensitivity (%) and specificity (%) of PCR-DGGE-S (culture was a reference method). Statistically significant differences (McNemar's test with continuity correction) in comparison to reference method are marked in bold (p < 0.05).