Table 2.

DNA extraction methods and percentage of PCR amplification success in obtaining COI DNA barcodes from mosquitoes.

Extraction method	No. plates / samples	Time per plate	Amplification success n (%)	Observations
1. Legs directly into molecular grade water and sonicated for 10 min	1 plate / 95 samples	30 min	41 (43.1%)	High sequencing failure (54 samples)
2. Legs directly into alkaline lysis buffer and sonicated for 10 min (Hotshot)	5 plates / 475 samples	1hr each plate	429 (90.3%)	Target length barcodes obtained
3. Abdomen processed using Qiagen kit	5 plates / 475 samples	Only 32 samples per 4hr session for DNA extraction for each plate	306 (64.4%)	Target length barcodes obtained. Vertebrate DNA amplified