Fig. 1.

Insufficient RFA increased FLOT1 and FLOT2 levels in HCC cells in vitro. a Western blot analysis of FLOT1 and FLOT2 levels in HCC cells. HepG2 cells were lysed 24 h after 10-min heat treatments at 41 °C, 44 °C, and 47 °C, HCCLM3 cells were lysed 24 h after 10-min heat treatments at 39 °C, 42 °C, and 45 °C. b Densitometry analyses depict relative changes in FLOT1 and FLOT2 expression. Data are presented as mean ± SD. Experiments were independently conducted three times; *P < 0.05 and **P < 0.01