Fig. 2.

Insufficient RFA increased FLOT1 and FLOT2 levels in residual cancer in vivo. a, b FLOT1 and FLOT2 mRNA and protein levels in heat-treated HCCLM3 and HepG2 residual cancer and sham-operated tissue were detected by western blot and RT-qPCR. c, d Immunohistochemical staining confirmed increased FLOT1 and FLOT2 protein levels in heat-treated HCCLM3 residual cancer, but not in heat-treated HepG2 residual cancer. Representative images are shown at 200× magnification. Data are presented as mean ± SD. Experiments were independently conducted three times; *P < 0.05 and **P < 0.01