Fig. 4.

Silencing FLOT1 and FLOT2 attenuated EMT-like changes in heat-treated HCCLM3 cells in vitro. a Morphologic changes observed in untreated and heat-treated HCCLM3 cells that were transfected with shVector, shFLOT1, shFLOT2, or shFLOT1 + 2. b, c RT-qPCR and western blot analyses of FLOT1, FLOT2, E-cadherin, N-cadherin, Vimentin, and Snail expression in control and heat-treated HCCLM3 cells that were transfected with shVector, shFLOT1, shFLOT2, or shFLOT1 + 2; scale bar = 50 µm. Data are presented as mean ± SD. Experiments were independently conducted three times; *P < 0.05, **P < 0.01 vs. the HCCLM3-WT group; ^#P < 0.05, ^##P < 0.01 vs. the heat-treated HCCLM3-shVector group