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. 2019 Mar 26;9:5214. doi: 10.1038/s41598-019-41536-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chronological changes in levels of intracellular activated CM-H₂DCFDA in the presence of angiotensin II receptor antagonists (Protocol 2). Acute response of H₂O₂ levels against angiotensin II stimulation in mesangial cells was significantly enhanced by administration of angiotensin II type 2 receptor antagonist (PD), but was significantly reduced by administration of angiotensin II type 1 receptor antagonist (RNH). Based on this result, angiotensin II-induced generation of oxidative stress in mesangial cells was suggested to be mediated by angiotensin II type 1 receptor. The error bar of each plot shows standard error. Abbreviations: Ang II, angiotensin II; M, mol/l; PD, angiotensin II type 2 receptor antagonist; RNH, angiotensin II type 1 receptor antagonist; s, second.