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. 2019 Mar 26;9:5141. doi: 10.1038/s41598-019-41672-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNA-seq results and Gene Ontology (GO) in top DEGs. (a) DETs and DEGs log₂ ratios of (Fragments Per Kilobase of transcript per Million mapped reads) FPKMs (JIMT1/SKBR3) meeting indicated criteria. Statistical testing was conducted by Cufflinks. (b) Three CD44 DETs that were DE at least 2-fold in JIMT1 relative to SKBR3 cells and their associated p-values as reported by Cufflinks for replicates. CD44 DE in JIMT1 and SKBR3 cells. (c) Gene ontology (GO) terms for top DE genes determined by DAVID¹⁵. Only p-values (as reported by DAVID) less than 0.05 are shown. (d) Two-tailed t-test of top-50 genes shown in (c) for each cell line.