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. 2019 Mar 26;9:5116. doi: 10.1038/s41598-019-41414-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of centrally nucleated fibers (CNF) among the different muscles and ages. (A) Percentage of CNF from the total amount of fibers. White columns correspond to wild-type (WT) mice whereas black columns correspond to McArdle (McA) mice. Only WT values from 8 wo mice are shown, as values WT values are similar among the three different ages. Each column represents the percentage of CNF resulting from the mean value obtained from 3 to 8 mice. The total number of mice used (N) is indicated at the bottom of each column. In each column between 491 (minimum) and 5,079 (maximum) fibers were counted (median 1682 fibers). Error bars correspond to standard deviation (SD). (B) Percentage of CNF in the different muscles of 8 and 70-week-old McA mice. For each age, muscles are represented together. Error bars correspond to SD. The non-parametric Kruskal-Wallis One-Way ANOVA with multiple comparisons test (Dunn’s test) was used for statistical analyses. Symbols: *p < 0.05.