Table 4.
Change of expression of genes related to methylation in oocytes following FSK and IBMX treatment.
| Feature ID | Annotations – database object name | Experiment – fold change* (original values) | Kal’s Z-test: P-value correction | Number of mapped reads before FSK and IBMX treatment | Number of mapped reads after FSK and IBMX treatment | Change of gene expression |
|---|---|---|---|---|---|---|
| MAT1A | S-Adenosylmethionine synthase | 7.4074074 | 0 | 27 | 200 | upregulated |
| BHMT2 | Betaine-homocysteine S-methyltransferase 2 | 1.8 | 0.685944354 | 5 | 9 | unchanged |
| MAT2A | S-Adenosylmethionine synthase | 1.3667011 | 0 | 2,907 | 3,973 | upregulated |
| BHMT | Betaine-homocysteine S-methyltransferase 1 | 1.2213115 | 0.416117351 | 122 | 149 | unchanged |
| AHCYL1 | Adenosylhomocysteinase like 1 | 1.1280168 | 0.108837606 | 953 | 1,075 | unchanged |
| AHCY | Adenosylhomocysteinase | 1.0471098 | 0.66715386 | 3,927 | 4,112 | unchanged |
| MAT2B | Methionine adenosyltransferase 2 subunit beta | 1.0045749 | 0.462293673 | 12,022 | 12,077 | unchanged |
| DNMT3L | DNA (cytosine-5)-methyltransferase 3-like | 1 | 1 | 0 | 0 | unchanged |
| SUV39H2 | Histone-lysine N-methyltransferase SUV39H2 | −1.0042614 | 0.785232355 | 2,121 | 2,112 | unchanged |
| DNMT3A | DNA (cytosine-5)-methyltransferase 3A | −1.0125589 | 0.979807318 | 645 | 637 | unchanged |
| MTR | Methionine synthase | −1.0139373 | 0.98656539 | 582 | 574 | unchanged |
| AHCYL2 | Adenosylhomocysteinase | −1.0439059 | 0.002982796 | 5,944 | 5,694 | downregulated |
| DOT1L | Histone-lysine N-methyltransferase,H3 lysine-79 specific | −1.0609936 | 0.040837852 | 2,157 | 2,033 | downregulated |
| TCL1B | T-cell leukemia/lymphoma 1B | −1.1037151 | 0 | 15,835 | 14,347 | downregulated |
| DNMT1 | DNA (cytosine-5)-methyltransferase 1 | −1.118876 | 0 | 18,476 | 16,513 | downregulated |
| DNMT3B | DNA (cytosine-5)-methyltransferase 3B | −1.1300284 | 2.04E-06 | 2,781 | 2,461 | downregulated |
| SUV39H1 | Histone-lysine N-methyltransferase SUV39H1 | −1.1617152 | 9.60E-13 | 4,145 | 3,568 | downregulated |
*When the ratio is less than 1, it is converted to its negative inverse.
The transcriptome data have been deposited in the DDBJ Sequence Read Archive (DRA) with accession number DRA006403.
Bold show upregulated. Italic show downregulated.