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. 2019 Mar 26;9:5165. doi: 10.1038/s41598-019-41621-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CCL21, CCR7 are expressed at injured sites of juvenile mice in the early phase of post injury. (A) Representative histology in juvenile WT mice with safranin O staining, H&E staining, immunostaining for type II collagen at 3 days, 1 week, and 2 weeks postoperatively. Scale bar: 100 µm. (B) Representative histology in juvenile CCR7^−/− mice with safranin O staining, H&E staining, immunostaining for type II collagen at 3 days, 1 week, and 2 weeks postoperatively. Scale bar: 100 µm. (C) Representative images for immunohistochemistry of CCL21 (red) in juvenile WT mice at 3 days, 1 week, and 2 weeks postoperatively. CCL21 was transiently expressed at the injury site in WT mice in the early phase of post injury (white arrow). Scale bar: 100 µm. (D) Representative images for immunohistochemistry of CCL21 (red) in juvenile CCR7^−/− mice at 3 days, 1 week, and 2 weeks postoperatively. CCL21 was transiently expressed at the injury site in CCR7^−/− mice in the early phase of post injury (white arrow). Scale bar: 100 µm.