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. 2019 Mar 26;9:5222. doi: 10.1038/s41598-019-41425-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Example of photographs taken from optic nerve cross-sections of wild-type and M1R−/− mice. at both age categories (A–D; scale bars 10 µm). The number of optic nerve axons was lower in old M1R−/− mice compared to younger mice of the same genetic background (D, **p < 0.01).